抑郁模型大鼠海马内特异性microRNAs的筛选以及柴胡疏肝散的干预作用(4)
Method： SD rats were randomly divided into 3 groups： the normal control group， the model control group and the Chaihu Shugan San group. The depression model was replicated by unpredictable chronic mild stress combined with separation. Behavioral changes of the rats were observed by Open，field test and sucrose solution consumption test， and the expression of microRNAs in hippocampus was assayed by microRNA micro，array.
Result： Compared with the normal control group， there were 13 specific miRNAs in hippocampus in the model control group with the expression difference of more than 2 times. Among them， down，regulating miRNAs included miR，298， miR，130b， miR，135a， miR，323， miR，503， miR，15b， miR，532， and miR，125a， and the up，regulation miRNAs included miR，7a， miR，212， miR，124， miR，139， and miR，182. Among the 13 specific miRNAs， miR，125a and miR，182 recovered to normal after intervention with Chaihu Shugan San in the Chaihu Shugan San group.
Conclusion： This study preliminarily found that 13 specific miRNAs in hippocampus are related to depression. Among them， miR，125a and miR，182 recover to normal after intervention with Chaihu Shugan San， which may be the target points of the antidepressant effect of Chaihu Shugan San. We shall further analyze the target genes and their mechanisms.
[Key words]microRNAs； depression model； hippocampus； Chaihu Shugan San
doi：10.4268/cjcmm201301027, 百拇医药(曹美群 陈德珩 张春虎 吴正治)
Result： Compared with the normal control group， there were 13 specific miRNAs in hippocampus in the model control group with the expression difference of more than 2 times. Among them， down，regulating miRNAs included miR，298， miR，130b， miR，135a， miR，323， miR，503， miR，15b， miR，532， and miR，125a， and the up，regulation miRNAs included miR，7a， miR，212， miR，124， miR，139， and miR，182. Among the 13 specific miRNAs， miR，125a and miR，182 recovered to normal after intervention with Chaihu Shugan San in the Chaihu Shugan San group.
Conclusion： This study preliminarily found that 13 specific miRNAs in hippocampus are related to depression. Among them， miR，125a and miR，182 recover to normal after intervention with Chaihu Shugan San， which may be the target points of the antidepressant effect of Chaihu Shugan San. We shall further analyze the target genes and their mechanisms.
[Key words]microRNAs； depression model； hippocampus； Chaihu Shugan San
doi：10.4268/cjcmm201301027, 百拇医药(曹美群 陈德珩 张春虎 吴正治)